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Molecular detection of Leptospira in urine samples of dogs from Kerman City, southeastern Iran
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H. Gonabadi   , E. Mohammadi * |
| Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran |
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Abstract: (211 Views) |
| Leptospirosis is a globally significant zoonotic disease, prevalent in tropical, subtropical, and temperate regions. The causative agents are spiral-shaped aerobic spirochetes of the genus Leptospira, classified into saprophytic (L. biflexa) and pathogenic (L. interrogans) species. Environmental and anthropogenic factors such as climate change, heavy rainfall, flooding, poor sanitation, improper waste management, and high reservoir host populations (e.g., rodents) contribute to transmission. Clinical manifestations in dogs range from mild fever to severe organ damage. Transmission occurs via direct contact with infected animals or through exposure to contaminated soil and water. Due to slow bacterial growth and absence of specific antibodies during early infection, culture and serological tests have limited diagnostic value. Polymerase chain reaction (PCR), with high sensitivity and specificity, offers a reliable alternative. In this study, urine samples from 50 dogs in Kerman, southeastern Iran, were tested for Leptospira DNA using PCR targeting the 16S rRNA gene. Leptospira DNA was detected in 6 out of 50 samples (12%). These results confirm the presence of Leptospira in the canine population of Kerman, highlighting a potential zoonotic risk. Enhanced surveillance and preventive measures are recommended to reduce public health impact. |
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| Keywords: Leptospira, Urine samples, PCR, Dog, Kerman City |
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Full-Text [PDF 427 kb]
(63 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2025/05/4
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