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Molecular and parasitological assessment of Theileria species and tick vector in sheep from Kerman, southeastern Iran
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R. Mahdavi   , A. Ahmadi * , M. Mazrouei , B. KheirKhah |
| Department of Basic Sciences, Faculty of Veterinary Medicine, Ardakan University, P.O. Box 184, Ardakan, Iran. & Biology and Animal Reproduction Science Research Institute, Ardakan University, P.O. Box 184, Ardakan, Iran. |
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Abstract: (25 Views) |
| This study investigated the prevalence of Theileria species in sheep from Kerman, utilizing a semi-nested PCR. Theileriosis, a tick-borne disease caused by protozoan parasites of the genus Theileria, poses significant threats to livestock health and agricultural productivity. The blood samples of 50 sheep were collected from the jugular vein of each sheep and transferred into tubes containing EDTA as an anticoagulant. Then, a thin blood smear was prepared for each sample for microscopic examination, and the remaining was stored at -20°C until molecular analysis. At the same time, the animals were thoroughly examined, and any ticks found on them were gathered into properly labeled specimen tubes. Molecular analysis confirmed that 22 samples (44%) were positive for Theileria, all categorically identified as Theileria ovis. This result signifies a marked contrast to the 20% prevalence rate observed through conventional microscopic examination, thereby highlighting the enhanced sensitivity of molecular diagnostic techniques.
Additionally, the tick species Hyalomma anatolicum was identified as a key vector in the transmission of Theileria, underscoring the ecological implications of these findings. Further comprehensive studies will be essential to evaluate the prevalence of Theileria species and the associated risk factors in sheep of the study area. The findings of this study emphasize the significance of employing advanced molecular diagnostic techniques to accurately determine the prevalence of Theileria species in sheep populations. The identified high prevalence of Theileria ovis, a pathogenic species, suggests the need for enhanced surveillance and implementation of targeted control measures to mitigate the impact of theileriosis on sheep health and productivity in the region.
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| Keywords: Theileria, Sheep, Semi-nested PCR, Kerman, Iran |
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Type of Study: Research |
Subject:
General
Received: 2025/05/20
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