H.h. Naji , W.a. Mohammed , A.d. Kuhdair , R.e. Sharhan , S.m. Abbas ,
Volume 4, Issue 1 (1-2024)
Abstract
This study was aimed at the possibility of using garlic (extract and CNP-G) in alleviating anemia induced by phenylhydrazine, in adult male rats.
Materials and methods: The chitosan nanoparticles containing garlic were prepared in the laboratory. Anemia was induced by phenylhydrazine intraperitoneal (20 mg/kg) for 2 consecutive days. A total of 60adult male albino rats were used in the present study, at the age of 2 months with body weight195±15gm were divided randomly to six equal groups (10 rats for each) and treated as follows for 8 weeks: CG: This group animals left without any treatment like negative control.T1: animals in this group was induced anemia and untreated as a positive group. T2: animals in this group are still normal but treated with a daily dose of 35.4mg/kg of extract garlic given orally by stomach tube. T3: animals in this group were induced anemia and treated with a daily dose of 35.4mg/kg of extract garlic given orally by stomach tube. T4: animals in this group still normal (no anemic) but treated with a daily dose of 35.4mg/kg of CNP-G given orally by stomach tube. T5: animals in this group were induced anemia and treated with daily dose 35.4mg/kg of chitosan nanoparticles containing garlic given orally by stomach tube. At the end of the experiment, all animals were sacrificed and blood samples(5ml) were collected directly from the heart by the cardiac puncture.
Results: Induction of anemia significantly (p<0.05) decreased PCV, hemoglobin concentration (Hb), and red blood cell count (RBC), while mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) significantly increased of anemic not treated rats compared to normal control rats. The total protein and albumin showed significantly decreased in anemic group but fibrinogen recorded a significant increase in anemic group as compared with control group. When rats received garlic extract and CNP-G reported increase in the blood electrolytes potassium and iron showed significantly increase in anemic group as compared with the control group. While, sodium and calcium recorded significantly decreased. After treatment by extracting and chitosan nanoparticles containing garlic the K+ and Fe+ increase in all treatment groups and Na+, Ca++ increased in all treatment groups. The result of hormone erythropoietin showed an increase in anemic group and decrease in treatment groups, while VB12 reported decrease in anemic group and increae in treatment groups. While, sodium and calcium recorded significant decreased. After treatment with extract and garlic loaded chitosan nanoparticles the K+ and Fe+ decreased in all treatment groups and Na+ , Ca++ increased in all treatment groups .
Conclusion: This study suggests may be use of CNP-G as antianemic and ability to prevent hemolysis best from garlic extract.
