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Detection of anti-cryptosporidium antibodies in colostrum of dairy cows in farms near Tehran: A study
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J. Abbasi *   , M.R. Mokhber Dezfouli , P. Shayan |
| Department of Animal and Poultry Health and Nutrition,Faculty of Veterinary Medicine,University ofTehran,Tehran ,Iran. & Institute of Biomedical Research, University of Tehran, Tehran, Iran |
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Abstract: (176 Views) |
| Cryptosporidiosis, a common disease caused by a protozoan that can be transmitted between animals and humans, was the focus of our comprehensive and meticulously conducted study. The aim of this study was to detect the anti-Cryptosporidium antibodies in the colostrum of cows from several dairy farms near Tehran. We began by collecting fecal samples from diarrheic calves suspected of having Cryptosporidium infection. These samples were then subjected to modified Ziehl-Neelsen staining to confirm the presence of Cryptosporidium based on morphological structure and staining characteristics of the parasite oocysts. Subsequently, oocysts were isolated and purified from the fecal samples using modified 55% sucrose flotation to ensure the extraction of pure oocysts for further analysis. To investigate the prevalence of Cryptosporidium infection in cows around Tehran, we collected 100 colostrum samples from four dairy farms in the region from the first milking of 100 cows of different ages. After removing the fat content from the colostrum samples, the detection of anti-Cryptosporidium antibodies was conducted using the indirect immunofluorescence assay (IFAT). One of the key findings of this study is the potential protective role of anti-Cryptosporidium antibodies in colostrum. Of the 100 colostrum samples collected, 64 tested positive for these antibodies, while 36 were negative. Calves that received colostrum from the negative samples experienced severe diarrhea and excretion of oocytes. In contrast, calves that received colostrum from the positive samples showed no clinical symptoms and a significant decrease in oocyst excretion. These results strongly suggest that anti-Cryptosporidium antibodies in colostrum can provide significant protection against Cryptosporidium infection in calves. This finding could potentially revolutionize the health and well-being of cattle populations. |
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| Keywords: Cryptosporidiosis, Colostrum, Indirect immunofluorescence method, Dairy cows. |
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Full-Text [PDF 414 kb]
(97 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2025/01/15 | Published: 2025/04/9
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