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Molecular diagnosis of Hymenolepis diminuta in human and rats in Babylon Province, Iraq
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A.M. Al-Musawi *   , S.M. Kareem , H. Alseady , T.A.H. AL-Rubeaye , S.S.H. Alshkarchy |
| Department of Parasitology, College of Veterinary Medicine, Al-Qasim Green University, Babylon. |
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Abstract: (488 Views) |
The current study's objective was to use molecular technique to detect the parasite in samples taken from human and rats in Babylon province. A total of 100 stool samples from human and 40 fecal samples from rats were collected during the beginning of July 2021 to the end of October and analysed by molecular methods. Molecular description of H. diminuta was achieved by gene sequence of internal transcribed spacer 1 (ITS1). The PCR confirmed the identification of the parasite by electrophoresis as well as DNA sequencing. The result showed that the infection rate in human was (5/100) 5%, while in rats was (11/40) 27.50%, DNA sequencing detected 5 positive samples of human were H. diminuta and 5 positive samples from rats were H. diminuta. We conclude from the present results that H. diminuta is similar in both human and rats, and thus it is a source of infection for human. We recommended to detect H. diminuta in other rodents as source of zoonotic infection.
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| Keywords: Hymenolepis diminuta, PCR, Human, Rats, ITS1, Sequence analysis |
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Full-Text [PDF 1135 kb]
(282 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/09/30 | Published: 2023/07/6
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