en Monitoring of viral pathogens in the main Goldfish (Carassius auratus) farms using Immunochemical and Molecular Biology techniques تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Diseases are among the most critical threats to the aquaculture industry so that their prevalence leads to major economic losses. Monitoring for viral pathogens in aquaculture has high importance due to their incurability, speed of transmission, the severity of pathogenesis, and difficulties of their diagnosis. According to reports of goldfish </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(<i>Carassius auratus</i>) </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">losses with suspected viral etiology in farms of Gilan province this study designed to inquiring about the existence of selected viral agents. Thus, the <a name="_Hlk111541733">spring viremia of carp (SVC) </a>and <a name="_Hlk111541743">Koi herpesvirus (KHV)</a> which are among the notifiable infectious diseases (OIE, 2019) were investigated by cell culture, PCR, RT-PCR, and immunofluorescent antibody test (IFAT). The results showed the absence of the RNA of <i>Rhabdovirus carpio</i> and the DNA of Koi herpesvirus in tested samples. Accordingly, 470 bp band related to SVCV and 292 bp band related to KHV were not confirmed in any of the samples in PCR test as well as neither Cytopathic effect in cell culture nor positive reaction in IFAT were observed. Hence, it seems that non-viral agents are the main causes of periodic population loss of goldfish in the surveyed farms and can be controlled and prevented by the health management procedures.</span></span></span></span></span></span></span> Goldfish (Carassius auratus), fish viruses, Koi herpesvirus (KHV), spring viremia of carp (SVC). 27 40 http://injvr.com/browse.php?a_code=A-10-26-1&slc_lang=en&sid=1 M. Ghasemi mohades@yahoo.com Yes Inland Waters Aquaculture Research center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Bandar Anzali, Iran. S.M.J. Zorriehzahra zorrieh@yahoo.com No Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Tehran, Iran J. Daghigh Roohi javad_daghigh@yahoo.com No Inland Waters Aquaculture Research center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Bandar Anzali, Iran. M. Faeed m_faeed@yahoo.com No Inland Waters Aquaculture Research center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Bandar Anzali, Iran. Z. Eynizadeh zahra.eynizadeh@gmail.com No Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran. M. Sayad Bourani mohammadborani@yahoo.com No Inland Waters Aquaculture Research center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Bandar Anzali, Iran. S. Haghighi Karsidani haghighikarsidani@yahoo.com No Department of fishery, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali, Iran S.F. Mirhashemi Nasab mirhashemi_v@yahoo.com No Inland Waters Aquaculture Research center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Bandar Anzali, Iran. M. Asgharnia mehrdad_asgharnia@yahoo.com No Inland Waters Aquaculture Research center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture research Education and Extension Organization (AREEO), Bandar Anzali, Iran.