en عمومى General پژوهشي Research <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">Piroplasmosis, caused by Theileria and Babesia species, is a major tick-borne disease affecting sheep health and productivity in Iran. This study evaluated alterations in the lipid profile, fat-soluble vitamins, and hematological and hepatic parameters of Afshari sheep naturally infected with piroplasm parasites. A cross-sectional survey was conducted in 2024 on 48 Afshari sheep from Kerman, Quchan, and Birjand regions. Animals showing clinical signs consistent with theileriosis or babesiosis and confirmed microscopically by Giemsa-stained blood smears were included. Blood samples were collected from the jugular vein for hematological and biochemical analysis. Complete blood counts, liver enzyme activities, lipid profiles, and concentrations of vitamins A, E, and K were determined. Data were analyzed using SPSS and R software, with significance set at p<0.05. Among the examined animals, 26 were infected and 22 served as uninfected controls. Infected sheep showed significant reductions in hematological indices (RBC, Hb, and WBC counts), total protein, triglycerides, and fat-soluble vitamin levels, accompanied by elevated liver enzyme activities (p<0.05). These alterations indicate anemia, hepatic dysfunction, and metabolic disturbances associated with piroplasmosis. Routine monitoring of liver enzymes and biochemical markers is recommended for early detection and management of infection in sheep flocks.</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span> Afshari sheep, piroplasmosis, hematology, lipid profile, liver enzymes 17 27 http://injvr.com/browse.php?a_code=A-10-74-2&slc_lang=en&sid=1 A. Ahmadi Amin-ahmadi@ardakan.ac.ir 10031947532846001560 10031947532846001560 Yes Department of Basic Sciences, Faculty of Veterinary Medicine, Ardakan University, P.O. Box 184, Ardakan, Iran. M. Mazrouei Sebdani mohammadmazrouei93@gmail.com 10031947532846001561 10031947532846001561 No Department of veterinary medicine, Baft Branch Islamic Azad University, Baft, Iran S.A. Hossini Bejaim HossiniBejaim@gmail.com 10031947532846001562 10031947532846001562 No Department of veterinary medicine, Baft Branch Islamic Azad University, Baft, Iran B. Shafiei Apoorvari Shafiei@gmail.com 10031947532846001563 10031947532846001563 No Department of veterinary medicine, Baft Branch Islamic Azad University, Baft, Iran M. Ebrahimi Gozalabadi Ebrahimi@gmail.com 10031947532846001564 10031947532846001564 No Department of veterinary medicine, Baft Branch Islamic Azad University, Baft, Iran F. Soltani Banavandi fatemehsoltanidvm @gmail.com 10031947532846001565 10031947532846001565 No Department of veterinary medicine, Baft Branch Islamic Azad University, Baft, Iran M. Borhani 10031947532846001566 10031947532846001566 No State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, China